The predictive ability of demographic and psychosocial risk factors, school-related characteristics, and service interventions on grade attainment among at-risk elementary school children in a truancy intervention program

The purpose of the current exploratory-descriptive retrospective study was to examine the demographic, school-related, and psychosocial risk factors among at-risk elementary school children (N = 12644) assessed at low and high levels of risk for continuing truancy. The sample was enrolled in 16 statewide program sites of a community-based truancy prevention program in Louisiana, which provided a letter and attendance monitoring for low-risk participants and intensive case management for high-risk participants. Intercorrelations among risk factors and referred services and on-time grade attainment were assessed for a subsample of the high-risk children (n = 6088). Binary logistic regression analyses were conducted to determine which correlates among the demographic, school-related, and psychosocial risk factors and services best predicted on-time grade level at 3 years out among a subsample of the high-risk children (n = 2864). Model fit to the data was modest. Findings showed that race and grade at program admission were significantly associated with on-time grade attainment at 3 years out. African-American participants were less likely to be on time for grade than participants not of African-American ethnicity. Participants in kindergarten were less likely to be on time than children in higher grades of elementary school. Children assessed as unmotivated by their teachers were less likely to be on time for grade at 3 years out than children who were not assessed as unmotivated. Other findings showed that participants who completed educational services were less likely to be on time for their grade than participants who did not receive educational services. Implications for social work practice, education, and research are discussed

Baton Rouge Collective Healing Initiative: Final Report

In response to recent and historic traumatic events that caused distrust and strained relationships between law enforcement and their communities, the U. S. Department of Justice, Office of Victims of Crime (OVC), selected five demonstration sites to invest in restorative and healing activities to repair community-police relationships. The International Association of Chiefs of Police (IACP) worked closely with the sites over the three-year grant period to improve relations through evidence-based interventions, technical assistance, and peer learning. The program, Law Enforcement and the Communities They Serve: Supporting Collective Healing in the Wake of Harm began in the selected cities, which included 1) Baton Rouge, Louisiana; 2) Houston, Texas; 3) Minneapolis, Minnesota; 4) Oakland, California; and 5) Rapid City, South Dakota. The purpose of Collective Healing was to foster meaningful dialogue and reconciliation among law enforcement agencies and the communities of color they serve, to increase the capacity of victim services programs, and to address officer health and wellness. Collective Healing programs were led by police departments and supported by victim assistance programs, behavioral health agencies, grassroots organizations, and academic partners. IACP provided technical assistance and training and conducted site visits to monitor accountability and effectiveness. The Baton Rouge Collective Healing Initiative was conducted from October 1, 2017, through September 30, 2020. The Baton Rouge Police Department (BRPD) served as the lead and fiscal agent for the project. BRPD hired a program manager to coordinate the partnership and complete grant activities. The original core members of the Baton Rouge Collective Healing Initiative included community partners who were vested in improving community-police relationships. The core partners included 100 Black Men of Metro Baton Rouge, the Baton Rouge Chapter of the National Association for the Advancement of Colored People (NAACP), Capital Area Human Services, LSU Social Research and Evaluation Center, and the Southern University, Center for Social Research

The Criminal Justice Response to the Opioid Crisis in East Baton Rouge Parish

This report describes: 1) the history and current state of the opioid crisis in East Baton Rouge Parish; 2) the current and planned efforts of the Innovative Prosecution Solutions for Combating Violent Crime and Illegal Opioids (IPS) grant to respond to the crisis; and 3) recommendations for criminal justice practitioners regarding the opioid crisis in our community. The goal of the IPS grant is to reduce opioid-related deaths by fostering interagency collaboration to disrupt local opioid supply chains, educate the community about the dangers of opioid abuse and addiction, and provide support for individuals with opioid use disorder. The rising prevalence of opioid-related overdose deaths and violence is a significant issue in East Baton Rouge Parish, Louisiana. From 2014 to 2018, opioid poisoning-related emergency department visits increased by approximately 45%. During this five-year period, heroin poisoning-related emergency department visits increased by approximately 30% and non-heroin opioid poisoning-related emergency department visits increased by approximately 59%. From 2018 to 2019, opioid overdose deaths in East Baton Rouge Parish increased by approximately 29%. Opioid overdose deaths have consistently accounted for nearly all overdose deaths in East Baton Rouge Parish during this timeframe. Early attempts to address the opioid crisis were challenging due to a lack of trust and cooperation among governmental agencies and community members most affected by the opioid epidemic. Criminal justice practitioners (e.g., law enforcement, correctional officials, defense attorneys, prosecutors, court officials) have an important role in reducing opioid-related addiction, opioid-related overdose deaths, and opioid-related violence within East Baton Rouge Parish communities. Individuals with substance use disorder are often fearful of interacting with law enforcement due to the belief that they will be arrested for their addiction, decreasing the likelihood that these individuals will reach out for critical treatment. Therefore, public health officials are often reluctant to include criminal justice practitioners in opioid use disorder-related efforts, despite their ability to engage in meaningful, productive partnerships. For these perceptions to change, criminal justice practitioners must demonstrate a commitment to addiction and overdose prevention by forming vital partnerships and supporting their partners\u27 prevention efforts to address this epidemic. By engaging in meaningful community partnerships, criminal justice practitioners can implement and support efforts to improve the lives of individuals with opioid use disorder and reduce opioid overdose deaths. The recommendations were informed by empirical research related to criminal justice and public health interventions concerning opioid use disorder

What is Working to Reduce Violent Crime? Evidence-Based Solutions

The purpose of this review is to examine and evaluate current approaches to reducing violent crime. The review reports on supportive techniques, strategies, programs, and practices that are evidence-informed to combat criminal activity, delinquency, and community disorder. Ineffective techniques, strategies, and programs are also included. The review provides potential strategies and programs that require additional empirical research to show whether they work. This review includes the integration of education, employment, social services, and public health services into efforts to reduce crime and ease the burden on law enforcement and justice systems. Recommendations for reducing violent crime are included

Louisiana Children\u27s Trust Fund Annual Report 2021-2022

Child abuse and neglect is a leading factor in the staggeringly high rates of child mortality in Louisiana. In 2017, Louisiana had 44,793 total referrals for child abuse and neglect of which 19,851 were investigated (CWLA, 2019). Child abuse and neglect can have multiple detrimental effects on a child’s physical, psychological, and behavioral health. Effective prevention efforts are critical to ensuring the immediate and long-term safety and well-being of children in Louisiana. Each year, LCTF selects high-quality proposals and funds a range of prevention efforts to protect children, strengthen family well-being, and educate the public about children’s safety. Local, national, and global events have greatly impacted our communities since 2020. These events included various social, economic, political, and medical crises. Events such as the COVID-19 pandemic, led to political, social unrest and the resulting business closures have caused a great deal of instability in communities around the state. It has taken a toll on individuals’ mental health and well-being. The Child Welfare Information Gateway (2022) says that family wellbeing is an important factor in reducing the likelihood of child abuse and neglect. These events do not impact everyone equally. However, difficult times for some can be felt more severely by other, even more so for the most marginalized populations in our community. Understanding the broader context of how policies and events have impacted our communities is important. These events have caused many to examine bias, resources available for families at risk, and how communities and individuals are making decisions for their children. It has also identified barriers that prevent some children and families from seeking the opportunities and accessing the resources they need to thrive

Dynamic ploidy changes drive fluconazole resistance in human cryptococcal meningitis.

BACKGROUND Cryptococcal meningitis (CM) causes an estimated 180,000 deaths annually, predominantly in sub-Saharan Africa, where most patients receive fluconazole (FLC) monotherapy. While relapse after FLC monotherapy with resistant strains is frequently observed, the mechanisms and impact of emergence of FLC resistance in human CM are poorly understood. Heteroresistance (HetR) - a resistant subpopulation within a susceptible strain - is a recently described phenomenon in Cryptococcus neoformans (Cn) and Cryptococcus gattii (Cg), the significance of which has not previously been studied in humans. METHODS A cohort of 20 patients with HIV-associated CM in Tanzania was prospectively observed during therapy with either FLC monotherapy or in combination with flucytosine (5FC). Total and resistant subpopulations of Cryptococcus spp. were quantified directly from patient cerebrospinal fluid (CSF). Stored isolates underwent whole genome sequencing and phenotypic characterization. RESULTS Heteroresistance was detectable in Cryptococcus spp. in the CSF of all patients at baseline (i.e., prior to initiation of therapy). During FLC monotherapy, the proportion of resistant colonies in the CSF increased during the first 2 weeks of treatment. In contrast, no resistant subpopulation was detectable in CSF by day 14 in those receiving a combination of FLC and 5FC. Genomic analysis revealed high rates of aneuploidy in heteroresistant colonies as well as in relapse isolates, with chromosome 1 (Chr1) disomy predominating. This is apparently due to the presence on Chr1 of ERG11, which is the FLC drug target, and AFR1, which encodes a drug efflux pump. In vitro efflux levels positively correlated with the level of heteroresistance. CONCLUSION Our findings demonstrate for what we believe is the first time the presence and emergence of aneuploidy-driven FLC heteroresistance in human CM, association of efflux levels with heteroresistance, and the successful suppression of heteroresistance with 5FC/FLC combination therapy. FUNDING This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377/Z/11/Z and the Daniel Turnberg Travel Fellowship

Identification of Functional Networks of Estrogen- and c-Myc-Responsive Genes and Their Relationship to Response to Tamoxifen Therapy in Breast Cancer

BACKGROUND: Estrogen is a pivotal regulator of cell proliferation in the normal breast and breast cancer. Endocrine therapies targeting the estrogen receptor are effective in breast cancer, but their success is limited by intrinsic and acquired resistance. METHODOLOGY/PRINCIPAL FINDINGS: With the goal of gaining mechanistic insights into estrogen action and endocrine resistance, we classified estrogen-regulated genes by function, and determined the relationship between functionally-related genesets and the response to tamoxifen in breast cancer patients. Estrogen-responsive genes were identified by transcript profiling of MCF-7 breast cancer cells. Pathway analysis based on functional annotation of these estrogen-regulated genes identified gene signatures with known or predicted roles in cell cycle control, cell growth (i.e. ribosome biogenesis and protein synthesis), cell death/survival signaling and transcriptional regulation. Since inducible expression of c-Myc in antiestrogen-arrested cells can recapitulate many of the effects of estrogen on molecular endpoints related to cell cycle progression, the estrogen-regulated genes that were also targets of c-Myc were identified using cells inducibly expressing c-Myc. Selected genes classified as estrogen and c-Myc targets displayed similar levels of regulation by estrogen and c-Myc and were not estrogen-regulated in the presence of siMyc. Genes regulated by c-Myc accounted for 50% of all acutely estrogen-regulated genes but comprised 85% (110/129 genes) in the cell growth signature. siRNA-mediated inhibition of c-Myc induction impaired estrogen regulation of ribosome biogenesis and protein synthesis, consistent with the prediction that estrogen regulates cell growth principally via c-Myc. The 'cell cycle', 'cell growth' and 'cell death' gene signatures each identified patients with an attenuated response in a cohort of 246 tamoxifen-treated patients. In multivariate analysis the cell death signature was predictive independent of the cell cycle and cell growth signatures. CONCLUSIONS/SIGNIFICANCE: These functionally-based gene signatures can stratify patients treated with tamoxifen into groups with differing outcome, and potentially identify distinct mechanisms of tamoxifen resistance

Amygdala 14-3-3ζ as a Novel Modulator of Escalating Alcohol Intake in Mice

Alcoholism is a devastating brain disorder that affects millions of people worldwide. The development of alcoholism is caused by alcohol-induced maladaptive changes in neural circuits involved in emotions, motivation, and decision-making. Because of its involvement in these processes, the amygdala is thought to be a key neural structure involved in alcohol addiction. However, the molecular mechanisms that govern the development of alcoholism are incompletely understood. We have previously shown that in a limited access choice paradigm, C57BL/6J mice progressively escalate their alcohol intake and display important behavioral characteristic of alcohol addiction, in that they become insensitive to quinine-induced adulteration of alcohol. This study used the limited access choice paradigm to study gene expression changes in the amygdala during the escalation to high alcohol consumption in C57BL/6J mice. Microarray analysis revealed that changes in gene expression occurred predominantly after one week, i.e. during the initial escalation of alcohol intake. One gene that stood out from our analysis was the adapter protein 14-3-3ζ, which was up-regulated during the transition from low to high alcohol intake. Independent qPCR analysis confirmed the up-regulation of amygdala 14-3-3ζ during the escalation of alcohol intake. Subsequently, we found that local knockdown of 14-3-3ζ in the amygdala, using RNA interference, dramatically augmented alcohol intake. In addition, knockdown of amygdala 14-3-3ζ promoted the development of inflexible alcohol drinking, as apparent from insensitivity to quinine adulteration of alcohol. This study identifies amygdala 14-3-3ζ as a novel key modulator that is engaged during escalation of alcohol use

A Role for the Unfolded Protein Response (UPR) in Virulence and Antifungal Susceptibility in Aspergillus fumigatus

Filamentous fungi rely heavily on the secretory pathway, both for the delivery of cell wall components to the hyphal tip and the production and secretion of extracellular hydrolytic enzymes needed to support growth on polymeric substrates. Increased demand on the secretory system exerts stress on the endoplasmic reticulum (ER), which is countered by the activation of a coordinated stress response pathway termed the unfolded protein response (UPR). To determine the contribution of the UPR to the growth and virulence of the filamentous fungal pathogen Aspergillus fumigatus, we disrupted the hacA gene, encoding the major transcriptional regulator of the UPR. The ΔhacA mutant was unable to activate the UPR in response to ER stress and was hypersensitive to agents that disrupt ER homeostasis or the cell wall. Failure to induce the UPR did not affect radial growth on rich medium at 37°C, but cell wall integrity was disrupted at 45°C, resulting in a dramatic loss in viability. The ΔhacA mutant displayed a reduced capacity for protease secretion and was growth-impaired when challenged to assimilate nutrients from complex substrates. In addition, the ΔhacA mutant exhibited increased susceptibility to current antifungal agents that disrupt the membrane or cell wall and had attenuated virulence in multiple mouse models of invasive aspergillosis. These results demonstrate the importance of ER homeostasis to the growth and virulence of A. fumigatus and suggest that targeting the UPR, either alone or in combination with other antifungal drugs, would be an effective antifungal strategy